Overexpression, refolding, and crystallization of an 80 kDa outer membrane protein
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چکیده
منابع مشابه
Overexpression and refolding of an 80-kDa iron transporter from the outer membrane of Escherichia coli.
IMAC [ 141 2.1. Pass solubilized material (about 45 ml) at a flow rate of 0.4 ml/min over Ni-NTA resin equilibrated with buffer A. 2.2. Wash Ni-NTA column at a flow rate of 0.4 ml/min with 120 ml of buffer A. 2.3. Elute N T R fusion protein at a flow rate of 0.2ml/min with 80ml of buffer B*. Combine peak fractions (1 3 ml) and add protease inhibitors. Save 1 ml for analysis. 2.4. Dilute remaini...
متن کاملExpression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27.
The cell envelope of the thermophilic bacterium Thermus thermophilus is multilayered and includes an outer membrane with integral outer membrane proteins that are not well characterized. The hypothetical protein TTC0834 from T. thermophilus HB27 was identified as a 22 kDa outer membrane protein containing eight predicted beta-strands. TTC0834 was expressed with an N-terminal His tag in T. therm...
متن کاملamplification and cloning of a gene encoding a 41 kda outer membrane protein (lipl41) of leptospira interrogans serovar canicola
background: leptospirosis has been recognized as an important reemerging infectious disease caused by pathogenic leptospira spp. a major challenge of this disease is the application of a basic research to improve diagnostic method. outer membrane proteins of leptospira are potential candidates that could be useful in diagnosis. among them the lipl41 is an immunogenic protein which is present on...
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Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...
متن کاملCloning, overexpression, purification, and immunobiology of an 85-kilodalton outer membrane protein from Haemophilus ducreyi.
We have identified an 85-kDa outer membrane protein that is expressed by all tested strains of Haemophilus ducreyi. Studies of related proteins from other pathogenic bacteria, including Haemophilus influenzae, Pasteurella multocida, Neisseria gonorrhoeae, Neisseria meningitidis, and Shigella dysenteriae, suggested a role for these proteins in pathogenesis and immunity. In keeping with the first...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1996
ISSN: 0108-7673
DOI: 10.1107/s0108767396093580